VR-837™
Download Genome Learn about the ATCC Genome PortalBK polyomavirus is propagated in HEL 299 cells (ATCC CCL-137). This strain was isolated in 1970 from the urine of a human kidney transplant patient in London.
- Product category
-
Viruses
- Product type
-
Animal virus
- Classification
- Polyomaviridae, Polyomavirus
- Common name
- BK Virus
- Genome sequenced strain
- Yes
- Isolation source
- Urine of a kidney transplant patient.
- Geographical isolation
- United Kingdom; London
- Storage conditions
-
-70°C or colder
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Required Products
These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.
Documentation
Certificate of Analysis Download
To download a certificate of analysis for BK polyomavirus (VR-837), enter the lot number exactly as it appears on your product label or packing slip.
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Certificate of Origin Download
To download a certificate of origin for BK polyomavirus (VR-837), enter the lot number exactly as it appears on your product label or packing slip.
Certificate of Origin Request
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This product sheet is not available online. We only provide this product sheet to customers who have purchased this biosafety level 3 product. If you purchased this product, please contact Technical Service for this product sheet.
Safety Data Sheet Download
Open the Safety Data Sheet for this product to download.
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Please check the Product Sheet and Safety Data Sheet Landing pagefor more information.
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
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Required Products
These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.
HEL 299
CCL-137
Eagle's Minimum Essential Medium (EMEM)
30-2003
Fetal Bovine Serum (FBS)
30-2020
Related Products
Quantitative Genomic DNA from BK Polyomavirus
VR-837DQ
Detailed product information
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Characteristics
- Comments
- Hemagglutination of 0.5% GPRBC. Virus MUST be freeze-thaw cycled 6 times for greatest infectivity.
- Mycoplasma contamination
- Not detected
Handling information
- Host
-
HEL 299 (ATCC CCL-137)
- Effects
-
cell degeneration; cell rounding; CPE
- Complete medium
-
EMEM (ATCC 30-2003) + 2% FBS (ATCC 30-2020)
- Temperature
-
37°C
- Atmosphere
-
95% Air, 5% CO2
- Incubation
- 14-23 days, a 5% CO2 in air atmosphere is recommended
- Recommendation for infection
- For best results, cells should be 24 to 48 hours old and 70-90% confluent (not 100% confluent)
- Handling notes
- Virus MUST be freeze-thaw cycled 6 times for greatest infectivity.
- Key abbreviations
-
°C, Degrees Celsius
CO2,Carbon dioxide
CPE, Cytopathic effect
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
Quality control specifications
- Volume
- 1.0 mL
- Verification method
-
Whole-genome Sequencing
History
- Deposited as
- BK virus
- Depositors
- G di Mayorca
- Type of isolate
- Human
- Year of origin
- 1970
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
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Frequently Asked Questions
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References
Curated Citations
Gardner SD, et al. New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet 1: 1253-1257, 1971. PubMed: 4104714
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