BK polyomavirus - VR-837 | ATCC (2024)

VR-837

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BK polyomavirus is propagated in HEL 299 cells (ATCC CCL-137). This strain was isolated in 1970 from the urine of a human kidney transplant patient in London.

Product category

Viruses

Product type

Animal virus

Classification
Polyomaviridae, Polyomavirus
Common name
BK Virus
Genome sequenced strain
Yes
Isolation source
Urine of a kidney transplant patient.
Geographical isolation
United Kingdom; London
Storage conditions

-70°C or colder

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Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Documentation

Product sheet

Certificate of Analysis Download

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Certificate of Origin Download

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This product sheet is not available online. We only provide this product sheet to customers who have purchased this biosafety level 3 product. If you purchased this product, please contact Technical Service for this product sheet.

Safety Data Sheet Download

Open the Safety Data Sheet for this product to download.

BK polyomavirus - VR-837 | ATCC (1)

If a requested product is not a hazardous chemical, or does not contain any hazardous chemicals, a SDS is not required and therefore will not be provided.

Please check the Product Sheet and Safety Data Sheet Landing pagefor more information.

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

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Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

HEL 299

CCL-137

Eagle's Minimum Essential Medium (EMEM)

30-2003

Fetal Bovine Serum (FBS)

30-2020

Related Products

Quantitative Genomic DNA from BK Polyomavirus

VR-837DQ

Detailed product information

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Characteristics

Comments
Hemagglutination of 0.5% GPRBC. Virus MUST be freeze-thaw cycled 6 times for greatest infectivity.
Mycoplasma contamination
Not detected

Handling information

Host

HEL 299 (ATCC CCL-137)

Effects

cell degeneration; cell rounding; CPE

Complete medium

EMEM (ATCC 30-2003) + 2% FBS (ATCC 30-2020)

Temperature

37°C

Atmosphere

95% Air, 5% CO2

Incubation
14-23 days, a 5% CO2 in air atmosphere is recommended
Recommendation for infection
For best results, cells should be 24 to 48 hours old and 70-90% confluent (not 100% confluent)
Handling notes
Virus MUST be freeze-thaw cycled 6 times for greatest infectivity.
Key abbreviations

°C, Degrees Celsius

CO2,Carbon dioxide

CPE, Cytopathic effect

EMEM, Eagle's Minimum Essential Medium

FBS, Fetal bovine serum

Quality control specifications

Volume
1.0 mL
Verification method

Whole-genome Sequencing

History

Deposited as
BK virus
Depositors
G di Mayorca
Type of isolate
Human
Year of origin
1970

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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Frequently Asked Questions

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References

Curated Citations

Gardner SD, et al. New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet 1: 1253-1257, 1971. PubMed: 4104714

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Telephone

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BK polyomavirus - VR-837 | ATCC (2024)

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